Coding

Part:BBa_K4812000:Design

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-07)


Scl1.1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 453
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 453
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 453
    Illegal SpeI site found at 183
    Illegal PstI site found at 995
    Illegal AgeI site found at 643
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 1211


Design Notes

Repeated sequences in the gene may make it difficult to synthesize the gene of interest. Gene may need to be fragmented and ordered separately. Fragments can then be assembled using standard methods, such as Gibson Assembly. Gene may need to be codon optimized for specific expression strains.


Source

Scl1.1 allele comes from the cell surface of group A Streptococcus(GAS), a Gram-positive bacterium, specifically serotype M1 strain MGAS6708 identical to SF370.

References

Xu, Y. et al. (2002) ‘Streptococcal SCL1 and SCL2 proteins form collagen-like triple helices’, Journal of Biological Chemistry, 277(30), pp. 27312–27318. doi:10.1074/jbc.m201163200.